●Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule(MCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule(MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Cell Adhesion Molecule(MCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Cell Adhesion Molecule(MCAM) in the samples is then determined by comparing the OD of the samples to the standard curve.●サイズ：96T●Alternative Names：CD146; MUC18; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen●Species：Mouse●Assay Type：Sandwich●Sensitivity：0.253 ng/mL●Standard：40 ng/mL●Detection range：0.63-40 ng/mL●Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids●Assay length：4.5h●Research Area：●この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）